| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.31[luc2P/GAL4UAS/Hygro] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 6107 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 带有启动子和Gal4上游激活序列的载体，用于研究使用不稳定荧光素酶的细胞信号。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pGL4.31[luc2P-GAL4UAS-Hygro]载体图谱](/uploads/载体图谱/pGL4.31[luc2P-GAL4UAS-Hygro]载体图谱.png)
载体简介
载体序列
LOCUS pGL4.31[luc2P GAL4UAS Hygro] 6107 bp ds-DNA circular SYN 27-JUL-2013
DEFINITION Vector with a promoter and a Gal4 upstream activating sequence for
studying cell signaling using destabilized luciferase.
ACCESSION DQ487213
VERSION .
KEYWORDS pGL4.31[luc2P GAL4UAS Hygro]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6107)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Monday, February 17, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6107
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
protein_bind 31..133
/bound_moiety="Gal4"
/note="GAL4 UAS"
/note="upstream activating sequence mediating
Gal4-dependent induction"
promoter 145..185
/note="Ad promoter"
/note="adenovirus major late promoter"
CDS 238..1887
/codon_start=1
/product="firefly luciferase"
/note="luciferase"
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
CDS 1891..2010
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/note="hPEST"
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
polyA_signal 2062..2183
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2377..2734
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2585..2720
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2765..3802
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
EVGRV"
polyA_signal 3826..3874
/note="synthetic polyadenylation signal"
rep_origin complement(4201..4789)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4989..5849)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 5954..6002
/note="synthetic polyadenylation signal"
misc_feature 6016..6107
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt
61 cctccgactc gagcggagta ctgtcctccg atcggagtac tgtcctccgc gaattccgga
121 gtactgtcct ccgaagacgc tagcgggggg ctataaaagg gggtgggggc gttcgtcctc
181 actctagatc tgcgatctaa gtaagcttgg caatccggta ctgttggtaa agccaccatg
241 gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc
301 gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc
361 tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt
421 cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc
481 agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct
541 gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc
601 cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag
661 aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc
721 caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc
781 gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt
841 accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat
901 gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg
961 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
1021 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
1081 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
1141 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
1201 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
1261 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
1321 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
1381 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
1441 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
1501 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
1561 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
1621 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
1681 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
1741 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
1801 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
1861 gccaagaagg gcggcaagat cgccgtgaat tctcacggct tccctcccga ggtggaggag
1921 caggccgccg gcaccctgcc catgagctgc gcccaggaga gcggcatgga tagacaccct
1981 gctgcttgcg ccagcgccag gatcaacgtc taaggccgcg actctagagt cggggcggcc
2041 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
2101 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
2161 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
2221 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
2281 ataaggatcc gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac catggcctga
2341 aataacctct gaaagaggaa cttggttagc taccttctga ggcggaaaga accagctgtg
2401 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2461 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
2521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
2581 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
2641 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
2701 aggaggcttt tttggaggcc taggcttttg caaaaagctc gattcttctg acactagcgc
2761 caccatgaag aagcccgaac tcaccgctac cagcgttgaa aaatttctca tcgagaagtt
2821 cgacagtgtg agcgacctga tgcagttgtc ggagggcgaa gagagccgag ccttcagctt
2881 cgatgtcggc ggacgcggct atgtactgcg ggtgaatagc tgcgctgatg gcttctacaa
2941 agaccgctac gtgtaccgcc acttcgccag cgctgcacta cccatccccg aagtgttgga
3001 catcggcgag ttcagcgaga gcctgacata ctgcatcagt agacgcgccc aaggcgttac
3061 tctccaagac ctccccgaaa cagagctgcc tgctgtgtta cagcctgtcg ccgaagctat
3121 ggatgctatt gccgccgccg acctcagtca aaccagcggc ttcggcccat tcgggcccca
3181 aggcatcggc cagtacacaa cctggcggga tttcatttgc gccattgctg atccccatgt
3241 ctaccactgg cagaccgtga tggacgacac cgtgtccgcc agcgtagctc aagccctgga
3301 cgaactgatg ctgtgggccg aagactgtcc cgaggtgcgc cacctcgtcc atgccgactt
3361 cggcagcaac aacgtcctga ccgacaacgg ccgcatcacc gccgtaatcg actggtccga
3421 agctatgttc ggggacagtc agtacgaggt ggccaacatc ttcttctggc ggccctggct
3481 ggcttgcatg gagcagcaga ctcgctactt cgagcgccgg catcccgagc tggccggcag
3541 ccctcgtctg cgagcctaca tgctgcgcat cggcctggat cagctctacc agagcctcgt
3601 ggacggcaac ttcgacgatg ctgcctgggc tcaaggccgc tgcgatgcca tcgtccgcag
3661 cggggccggc accgtcggtc gcacacaaat cgctcgccgg agcgcagccg tatggaccga
3721 cggctgcgtc gaggtgctgg ccgacagcgg caaccgccgg cccagtacac gaccgcgcgc
3781 taaggaggta ggtcgagttt aaactctaga accggtcatg gccgcaataa aatatcttta
3841 ttttcattac atctgtgtgt tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg
3901 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
3961 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
4021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
4081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
4141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
4201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
4261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
4321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
4381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
4441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
4501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
4561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
4621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
4681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
4741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
4801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
4861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
4921 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
4981 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
5041 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
5101 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
5161 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
5221 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
5281 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
5341 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
5401 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
5461 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
5521 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
5581 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
5641 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
5701 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
5761 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
5821 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
5881 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
5941 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
6001 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
6061 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctct
//
